A multi-centre international study of salivary hormone oestradiol and progesterone measurements in ART monitoring
Denny Sakkas, Colin M Howles, Leslie Atkinson, Andrea Borini, Ernesto A Bosch, Crystal Bryce, Monica Cattoli, Alan BCopperman, Astrid Finet de Bantel, Brian French, Jan Gerris, Steve W Granger, Veronika Grzegorczyk-Martin, Joseph A Lee, Michael J Levy, Marla J Matin, Sara Somers, Eric A Widra, Michael M Alper
RBMO VOLUME 42, ISSUE 2, P421-428, FEBRUARY 01, 2021, Published:October 23,2020 DOI:https://doi.org/10.1016/j.rbmo.2020.10.012
Ovarian stimulation during IVF cycles involves close monitoring of oestradiol, progesterone and ultrasound measurements of follicle growth. In contrast to blood draws, sampling saliva is less invasive. Here, a blind validation is presented of a novel saliva-based oestradiol and progesterone assay carried out in samples collected in independent IVF clinics.
Concurrent serum and saliva samples were collected from 324 patients at six large independent IVF laboratories. Saliva samples were frozen and run blinded. A further 18 patients had samples collected more frequently around the time of HCG trigger. Saliva samples were analysed using an immunoassay developed with Salimetrics LLC.
In total, 652 pairs of saliva and serum oestradiol were evaluated, with correlation coefficients ranging from 0.68 to 0.91. In the European clinics, a further 237 of saliva and serum progesterone samples were evaluated; however, the correlations were generally poorer, ranging from -0.02 to 0.22. In the patients collected more frequently, five out of 18 patients (27.8%) showed an immediate decrease in oestradiol after trigger. When progesterone samples were assessed after trigger, eight out of 18 (44.4%) showed a continued rise.
Salivary oestradiol hormone testing correlates well to serum-based assessment, whereas progesterone values, around the time of trigger, are not consistent from patient to patient.